Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR
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چکیده
منابع مشابه
Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR.
(13)C NMR is a powerful tool for monitoring metabolic fluxes in vivo. The recent availability of automated dynamic nuclear polarization equipment for hyperpolarizing (13)C nuclei now offers the potential to measure metabolic fluxes through select enzyme-catalyzed steps with substantially improved sensitivity. Here, we investigated the metabolism of hyperpolarized [1-(13)C(1)]pyruvate in a widel...
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In the heart, detection of hyperpolarized [(13)C]bicarbonate and (13)CO(2) by magnetic resonance (MR) after administration of hyperpolarized [1-(13)C]pyruvate is caused exclusively by oxidative decarboxylation of pyruvate via the pyruvate dehydrogenase complex (PDH). However, liver mitochondria possess alternative anabolic pathways accessible by [1-(13)C]pyruvate, which may allow a wider diagno...
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The production of glycolytic end products, such as lactate, usually evokes a cellular shift from aerobic to anaerobic ATP generation and O2 insufficiency. In the classical view, muscle lactate must be exported to the liver for clearance. However, lactate also forms under well-oxygenated conditions, and this has led investigators to postulate lactate shuttling from non-oxidative to oxidative mus...
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(13)C-detected experiments are still limited by their inherently lower sensitivity, as compared to the equivalent (1)H-detected experiments. Improving the sensitivity of (13)C detection methods remains a significant area of NMR research that may provide better means for studying large macromolecular systems by NMR. In this communication, we show that (13)C-detected experiments are less sensitiv...
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J. Bargon, J. Bernarding, R. Rizi, H-W. Spiess, K. Münnemann, M. Roth, and U. Bommerich Institute of Physical and Theoretical Chemistry, University of Bonn, Bonn, NRW, Germany, Institute of Biometry, University of Magdeburg, Magdeburg, Germany, Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States, NMR, Max-Planck Institute of Polymers, Mainz, Germany, I...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2007
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0706235104